Recombinant:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Indirect Immunoperoxidase Assay:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Liquid Chromatography with Mass Spectroscopy:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Adhesive:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Transferring:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Software:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Targeted Proteomics:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
Cell Surface Hydrophobicity:Article Title: Protocol for high-throughput, automated, solid-phase extraction of sphingolipids from human urine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Acetonitrile (ACN, LC–MS grade) Fisher Chemical Cat# A955-4 Ammonium formate Sigma-Aldrich Cat# 70221 Formic acid (Optima LC–MS grade) Fisher Chemical Cat# A117-50 Isopropanol (IPA, LC–MS grade) Fisher Chemical Cat# A464-4 Methanol (MeOH, LC–MS grade) Fisher Chemical Cat# A456-4 Methyl tert-butyl ether (MTBE) Sigma-Aldrich Cat# 34875 PBS, pH 7.4 Thermo Fisher Cat# 10010023 1-Propanol (PrOH, LC–MS grade) Fisher Chemical Cat# A399-4 Sphingolipid internal standards Avanti Polar Lipids See recipe Water (H 2 O, LC-MS grade) Fisher Chemical Cat# W6-4 Critical commercial assays/Consumables Agilent Captiva EMR-Lipid 96-well plate Agilent Technologies Cat# 5191-4110 96-well deep-well plates (1 mL) Eppendorf or VWR e.g., VWR Cat# 82054-142 Captiva 96-deep well collection plate (1 mL) Agilent Cat# A696001000 Adhesive sealing foil Thermo Fisher Cat# AB0625 Positive-displacement pipette tips Gilson Depends on model Autosampler vials with inserts or polypropylene 96-well LC plate Agilent Cat# 5182-0554 and 5183-2086 or Cat# 5042-8502 Experimental models: Organisms/strains Human urine samples University of Utah IRB-approved protocol NCT03335865 Software and algorithms Agilent MassHunter Acquisition (for QQQ/QTOF) Agilent Technologies Version 10.1 Agilent MassHunter Quantitative Analysis Agilent Technologies Version B.07.00 Prism 10 Graphpad N/A Excel Microsoft N/A Other Tecan Resolvex A200 positive-pressure manifold Tecan N/A Agilent 6490 Triple Quadrupole LC–MS Agilent Technologies G6490A Agilent 1290 Bin Pump Agilent Technologies G4220A Agilent 1290 Sampler Agilent Technologies G4226A Agilent Column Compartment Agilent Technologies G1316A Waters Acquity UPLC CSH C18 column (2.1 3 100 mm, 1.7 μm) Waters Cat# 186005297 Nitrogen evaporator (e.g., Biotage, Organomation) Various N/A Sonicator VWR or Branson N/A Benchtop centrifuge Eppendorf N/A − 80 ◦ C freezer Thermo Fisher N/A 2 STAR Protocols 7, 104644, September 18, 2026 ..
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